Plant tissue culture is a method that enables the regeneration or propagation of plant parts or whole plants by culturing plant protoplasts, cells, tissues, or organs under controlled conditions in vitro. Among these techniques, anther culture, meristem culture, shoot tip culture, ovule culture, and embryo culture are widely used in vitro techniques.

Embryo culture, defined as the cultivation of embryos isolated from seeds or seed primordia of higher plants on sterile nutrient media, is utilized for purposes such as the rapid development of new varieties, the creation of varieties more resistant to diseases, pests, and abiotic stresses, and enabling hybridization efforts hindered by incompatibility between plant genera and species. In grapevine, embryo culture is employed for objectives such as developing new grape varieties, propagating seedless grape cultivars, and creating varieties resistant to diseases, pests, and diverse ecological conditions. However, despite extensive research on embryo rescue in viticulture, the number of newly developed cultivars remains limited. Breeding new grape varieties through embryo rescue is still a challenging and long-term technique, requiring a patient and dedicated approach from grape breeders. This compilation will provide current and comprehensive insights into the embryo rescue technique in grape breeding, discussing its applications and practices.