Objective: This study aimed to evaluate the effectiveness of a blood transfusion training program for nurses by assessing their ability to accurately perform critical pre-transfusion procedural steps through a virtual game simulation.

Materials and Methods: This study was conducted using a single-group pretest–posttest quasi-experimental design. The study population consisted of 213 nurses working in a public hospital in İzmir between January and June 2025, and the sample included 169 nurses who agreed to participate. Data were collected using a Descriptive Information Form and a Virtual Game Simulation Program. Pretest data were obtained through face-to-face interviews prior to the training, while posttest data were collected via the same simulation program two weeks after the training. Statistical analyses were performed using SPSS for Windows version 25.0.

Results: Of the participants, 88.8% were female, 55% were aged between 18 and 35 years, and 82.2% held a bachelor’s degree. Following the training, improvements were observed in all evaluated steps of the blood transfusion procedure. The mean score increased from 9.28±2.05 in the pretest to 11.79±0.48 in the posttest, with the difference being statistically significant (p<0.001).

Conclusion: The findings of this study demonstrated that the training provided significantly improved nurses’ accuracy in performing pre-transfusion procedural steps. The virtual game simulation was utilized to evaluate these critical steps within a safe, repeatable, and standardized environment.