When the basic mechanisms important for the survival of living organisms were examined, it was found that enzymes from the protein biomolecule family had critical roles. Enzymes are today's most important biomolecules in many areas, from basic life functions to diagnosis, the development of treatment tools and therapies, determination of the efficacy and toxicity of therapeutics, and industrial product development for the production of cleaning products and cosmetics. Enzymes played a particularly significant role in the emergence of the field of biochemistry from organic chemistry. From the first discovery of enzymes to their classification, the field of molecular biology was established as an interdisciplinary field as combination of genetics, physics, and chemistry with the development of biochemistry. The critical role of enzymes has also manifested itself in this new field. With the development of computer technology, programming has emerged as a key field, and the development of data collection, processing, and mining has led to the emergence of the field of bioinformatics. Today, the integration of all these fields with the contribution of bioinformatics, in enzyme-focused studies, clarifies the critical role of enzymes from basic sciences to health.